Effect of Foliar Treatment of Micronutrients (Iron, Zinc, and Manganese) on Nitrogen Yield and Biological Fixation of Bami Alfalfa (Medicago Sativa L.) With Inoculation of Bacteria in Kerman

Document Type : Original Article


Department of Agriculture, Rafsanjan Branch, Islamic Azad University, Rafsanjan, Iran


This study was carried out aimed to evaluate the effect of foliar treatment of micronutrients including iron, zinc, and manganese on nitrogen yield and biological fixation with inoculation of bacteria in the Bami alfalfa in order to obtain a cost-effective product within Kerman in 2013 and was implemented for two years in Shahid Zendeh Rood agricultural education center in Kerman. The project was studied in an area of 2500 square meters using a split-plot design, with four replications and with the foliar treatment of micronutrients in 5 levels (a level as a control), and inoculation of bacteria in 3 levels ( non-inoculated as a control group). In all treatments, the distance between the lines was 30 cm, and all effects of the main factors and sub-factors on agronomic traits were analyzed statistically. Qualitative traits included plant height, yield, growth, and nitrogen fixation, and quantitative traits included the amount of protein, chlorophyll and pigments, digestibility, and the percentage of iron, zinc, manganese, nitrogen, phosphorus, and potassium. Overall, the survey results indicate that foliar treatment and inoculation had a significant impact on chlorophyll content and yield, and increased the amount of chlorophyll, protein, and the yield of Bami alfalfa.


Volume 4, Issue 1
June 2020
Pages 51-59
  • Receive Date: 08 February 2020
  • Revise Date: 15 April 2020
  • Accept Date: 03 June 2020
  • First Publish Date: 10 July 2020